PAC-1 (VO-100)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$450	Check With Us
500mg	$850	Check With Us
1g	$1275	Check With Us

: [email protected] [email protected]

Cat #: V0025 CAS #: 315183-21-2 Purity ≥ 98%

Description: First procaspase-activating compound (PAC-1; VO 100) is a potent small-molecule activator of procaspase-3 that directly catalyzes the maturation of procaspase-3 to the active caspase-3 by inducing the cleavage of procaspase-3 in a time-dependent manner.

References: Wang F, et al. A novel small-molecule activator of procaspase-3 induces apoptosis in cancer cells and reduces tumor growth in human breast, liver and gallbladder cancer xenografts. Mol Oncol. 2014 Dec;8(8):1640-52.

Top Publications Citing Invivochem Products

Product Catalog 2022

Guide to Product Handling

Publications Citing InvivoChem Products

Product Promise

Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	392.49
Molecular Formula	C23H28N4O2
CAS No.	315183-21-2
Storage	-20℃ for 3 years in powder formrrrrrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 78 mg/mL (198.73 mM)rrrrrr
	Water:<1 mg/mL (slightly soluble or insoluble)rrrrrr
	Ethanol: 16 mg/mL (40.76 mM)
Solubility In Vivo	30% PEG400+0.5% Tween80+5% Propylene glycol: 30mg/mL
SMILES Code	O=C(N/N=C/C1=CC=CC(CC=C)=C1O)CN2CCN(CC3=CC=CC=C3)CC2
Synonyms	PAC-1; PAC1; PAC 1; VO-100; VO100; VO 100; procaspase activating compound-1

Protocol	In Vitro	PAC-1 activates procaspase-3 with an EC50 of 2.08 μM. PAC-1 exhibits an enhanced zinc chelating ability (EC50= 7.08 μM). PAC-1 induces leukemia cell death with IC50 of 4.03 μM, which is consistent with the values reported by other investigators. PAC-1 treatment also results in death of other malignant cells in a concentration-dependent manner with IC50s ranging from 4.03 to 53.44μM. The overall mean IC50 in the fifteen malignant cell lines is 0.88 mM for WF-210 and 19.40 μM for PAC-1. In contrast, the sensitivity of the normal human cells (PBL, L-02, HUVEC and MCF 10A) to WF-210 is 2.6-fold lower (mean IC50=412.34 μM) than PAC-1 (mean IC50=158.29 μM).
Protocol	In Vivo	As shown in both PAC-1 and WF-210 significantly inhibits the growth of Hep3B tumor xenografts.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5478 mL	12.7392 mL	25.4784 mL	50.9567 mL
5mM	0.5096 mL	2.5478 mL	5.0957 mL	10.1913 mL
10mM	0.2548 mL	1.2739 mL	2.5478 mL	5.0957 mL
20mM	0.1274 mL	0.6370 mL	1.2739 mL	2.5478 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.