HA14-1
This product is for research use only, not for human use. We do not sell to patients.
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| Size | Price | Stock |
|---|---|---|
| 500mg | $450 | Check With Us |
| 1g | $660 | Check With Us |
| 5g | $1670 | Check With Us |
: Cat #: V0007 CAS #: 65673-63-4 Purity ≥ 98%
Description: HA14-1 is a novel, potent and non-peptidic inhibitor of a B-cell lymphoma 2 (Bcl-2) surface pocket with IC50 of ~9 μM.
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| Molecular Weight (MW) | 409.23 |
|---|---|
| Molecular Formula | C17H17BrN2O5 |
| CAS No. | 65673-63-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 82 mg/mL (200.4 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: 82 mg/mL (200.4 mM) | |
| Solubility In Vivo | 1% DMSO+30% polyethylene glycol+1% Tween 80: 30 mg/mL |
| SMILES Code | O=C(C1=C(N)OC2=C(C=C(Br)C=C2)[C@H]1[C@H](C#N)C(OCC)=O)OCC |
| Synonyms | HA 141, HA-141, HA141 |
| Protocol | In Vitro | HA14-1 is a nonpeptidic ligand of a Bcl-2 surface pocket. HA14-1 induces the activation of Apaf-1 and caspases, possibly by binding to Bcl-2 protein and inhibiting its function. The interaction of HA14-1 with the Bcl-2 surface pocket appeared to be specific for the chemical structure of HA14-1 as a series of synthetic analogs derived from HA14-1 containing various modifications are found to have widely different Bcl-2 binding activities. To examine the effect of HA14-1 on cell viability, HL-60 cells are treated with various concentrations of HA14-1 for 4 h. HA14-1 induces the death of HL-60 cells in a dose-dependent manner. At 50 μM, HA14-1 causes the lost of viability in more than 90% of the cells. |
|---|---|---|
| In Vivo | Swiss nude mice are challenged with BeGBM cells (104 injected s.c.). HA14-1 (400 nmol) in 100 μL free RPMI 1640-50% DMSO, or the carrier alone, is given at the site of injection once weekly from day 2 following cell injection. HA14-1 treatment does not have any significant effect on the growth of glioblastoma tumors in immunodeficient mice as monitored twice weekly by measuring the volume of the expanding tumors. Moreover, no gross organ toxicity or weight loss can be detected in mice receiving such treatment. To analyze whether HA14-1 treatment might enhance the efficiency of another antitumoral treatment, Swiss nude mice challenged with human glioblastoma multiforme cells are also given i.p. low doses of Etoposide (2.5 mg/kg in 200 μL of 0.9% NaCl 5 days a week from day 2 following cell injection) together with HA14-1 or mock treatment. Whereas Etoposide treatment is insufficient by itself to restrain the growth of glioblastoma cells, its combination with HA14-1 leads to a significant restrain on tumor growth as judged by the ability of the combined treatment to increase the doubling time of the tumor volume. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.4436 mL | 12.2181 mL | 24.4361 mL | 48.8723 mL |
| 5mM | 0.4887 mL | 2.4436 mL | 4.8872 mL | 9.7745 mL |
| 10mM | 0.2444 mL | 1.2218 mL | 2.4436 mL | 4.8872 mL |
| 20mM | 0.1222 mL | 0.6109 mL | 1.2218 mL | 2.4436 mL |
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Method for preparing in vivo formulation:
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PEG300, mix and clarify, next add
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Tween 80,mix and clarify, next add
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