5-Aminofluorescein

This product is for research use only, not for human use. We do not sell to patients.

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Cat #: V30874 CAS #: 3326-34-9 Purity ≥ 99%

Description: 5-Aminofluorescein (5-AF) is a novel fluorescence marker that covalently binds to human serum albumin (AFL-HSA). It is a pH-sensitive biomaterial used as a targeted prodrug for synthesis of FA-FITC-Arg-PTX and FA-5AF- Glu-PTX.

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Molecular Weight (MW)	347.32
Molecular Formula	C₂₀H₁₃NO₅
CAS No.	3326-34-9
Synonyms	5-AF

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.8792 mL	14.3959 mL	28.7919 mL	57.5838 mL
5mM	0.5758 mL	2.8792 mL	5.7584 mL	11.5168 mL
10mM	0.2879 mL	1.4396 mL	2.8792 mL	5.7584 mL
20mM	0.1440 mL	0.7198 mL	1.4396 mL	2.8792 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.