666-15
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $950 | Check With Us |
| 500mg | $1450 | Check With Us |
| 1g | $2175 | Check With Us |
: Cat #: V5228 CAS #: 1433286-70-4 Purity ≥ 98%
Description: 666-15 is a novel, cell-permeable, potent and selective inhibitor of CREB (cAMP-response element binding protein)-mediated gene transcription with IC50 of 81 nM.
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- Related Biological Data
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| Molecular Weight (MW) | 620.52 |
|---|---|
| Molecular Formula | C33H31Cl2N3O5 |
| CAS No. | 1433286-70-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| SMILES Code | O=C(C1=C(OCCCN)C=C2C=CC=CC2=C1)NCCOC3=C(C(NC4=CC=C(Cl)C=C4O)=O)C=C5C=CC=CC5=C3.[H]Cl |
| Synonyms | 66615; 666 15; 666-15 |
| Protocol | In Vitro | MCF-7 cells, it exhibits robust activity as well with GI50 of 0.47 and 0.31 μM. 666-15 is also found to be a rather weak inhibitor of CREB-CBP interaction with IC50 of 18.27 μM. 666-15 inhibits CREB’s transcription activity in living cells independent of direct CREB or CBP binding interaction. 666-15 is very potent in inhibiting CREB’s transcription activity. 666-15 also inhibits endogenous CREB target gene expression, the transcript level of nuclear receptor related 1 protein (Nurr1/NR4A2). |
|---|---|---|
| In Vivo | 666-15 (10 mg/kg; IP; once a week; for 11 weeks) alone can play a good role in inhibiting the growth of breast cancer, and the combination with RP-56976 (DOC) shows a better effect. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.6116 mL | 8.0578 mL | 16.1155 mL | 32.2310 mL |
| 5mM | 0.3223 mL | 1.6116 mL | 3.2231 mL | 6.4462 mL |
| 10mM | 0.1612 mL | 0.8058 mL | 1.6116 mL | 3.2231 mL |
| 20mM | 0.0806 mL | 0.4029 mL | 0.8058 mL | 1.6116 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.