3-Methyltoxoflavin
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1400 | To Be Confirmed |
| 500mg | $1950 | To Be Confirmed |
| 1g | $2925 | To Be Confirmed |
: Cat #: V9527 CAS #: 32502-62-8 Purity ≥ 98%
Description: 3-Methyltoxoflavin is a novel inhibitor of Hsp90.
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| Molecular Weight (MW) | 207.19 |
|---|---|
| Molecular Formula | C8H9N5O2 |
| CAS No. | 32502-62-8 |
| SMILES Code | O=C(C1=NC(C)=NN(C)C1=N2)N(C)C2=O |
| Synonyms | 3-Methyltoxoflavin; 3 Methyltoxoflavin; 3Methyltoxoflavin; GNF-Pf-2272 |
| Protocol | In Vitro | 3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM. 3-Methyltoxoflavin is toxic in a panel of human glioblastoma cell lines. From the screen, 3-Methyltoxoflavin emerges as the most cytotoxic inhibitor of PDI. Bromouridine labeling and sequencing (Bru-seq) of nascent RNA reveals that 3-Methyltoxoflavin induces Nrf2 antioxidant response, ER stress response, and autophagy. Specifically, 3-Methyltoxoflavin upregulates heme oxygenase 1 and SLC7A11 transcription and protein expression and represses PDI target genes such as TXNIP and EGR1. Interestingly, 3-Methyltoxoflavin-induced cell death does not proceed via apoptosis or necrosis, but by a mixture of autophagy and ferroptosis. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 4.8265 mL | 24.1324 mL | 48.2649 mL | 96.5298 mL |
| 5mM | 0.9653 mL | 4.8265 mL | 9.6530 mL | 19.3060 mL |
| 10mM | 0.4826 mL | 2.4132 mL | 4.8265 mL | 9.6530 mL |
| 20mM | 0.2413 mL | 1.2066 mL | 2.4132 mL | 4.8265 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.